Farzana Alam

PhD Overview

Cell-free protein synthesis (CFPS) has emerged as an alternative to using living cells for producing target proteins. It is particularly effective for synthesizing toxic or hard-to-express proteins, such as membrane proteins, and offers the advantage of a fully controlled reaction environment. Eukaryotic lysate-based CFPS systems are better suited for producing proteins that require post-translational modifications because they naturally contain the necessary components. In my PhD research, I am working on developing a method to successfully express glycoproteins in a cell-free system by utilizing endogenous microsomes derived from Pichia pastoris cell extracts.